Type 1 CALR mutation allele frequency correlates with CD34/CXCR4 expression in myelofibrosis-type megakaryocyte dysplasia: A mechanism of disease progression?

Calreticulin ( CALR ) mutations drive myeloproliferative neoplasms (MPNs). A 52-bp deletion (type 1) and a 5-bp insertion (type 2) are the most common mutations

Recently, it was reported that expression of S100A8, a calciumbinding protein implicated in proliferation, differentiation, and apoptosis of several cell types, is increased in type 1 CALR-mutated MPN-model cells [6].It is suggested the key to the divergent S100A8 concentrations results from an epigenetic alteration, due to the different methylation status of CALR harboring type 1 mutation.
We hypothesized that CALR type 1 mutation could epigenetically de-regulate the expression of proteins involved in the progression of MPNs other than S100A8.To test this hypothesis, we analyzed the influence of CALR mutation sub-types on the chemokine receptor CXCR4, a key regulator of homing, retention, and quiescence of hematopoietic stem cells (HSCs).The choice of CXCR4 was determined by data indicating that in myelofibrosis (MF) CXCR4 transcription is regulated by abnormal methylation of the CXCR4 promoter [7].Reduced CXCR4 surface expression on CD34-positive hematopoietic stem and progenitor cells (CD34/ CXCR4) is associated with a briefer interval to disease progression, blast transformation, and death [8].
We interrogated a cohort of 188 subjects with CALR mutation classified as myelofibrosis-type megakaryocyte dysplasia (MTMD), including 48 with clonal megakaryocyte dysplasia with isolated thrombocytosis (CMD-IT-falling into the category of MPNunclassifiable according to the WHO classification [9]), 54 with prefibrotic myelofibrosis (pre-MF) and 86 with overt myelofibrosis (overt-MF).Diagnoses of pre-MF and overt-MF were based on operative WHO diagnostic criteria at the time of their first visit and re-classified according to 2022 revised criteria [10].CMD-IT subjects were otherwise classified using adjudicated criteria [11].
We derived CD34/CXCR4 expression values and healthcare data by reviewing the results of subjects referred to the Center for the Study of Myelofibrosis at the IRCCS Policlinico S. Matteo Foundation in Pavia.The inclusion of subjects in the institutional database had been approved by the IRCCS Policlinico S. Matteo Foundation's Institutional Ethics Committee and subjects had given their written informed consent (Reference 20110004143 of the 26.9.2011).
CALR mutation was assayed by PCR amplification and capillary gel electrophoresis starting from granulocyte DNA.CALR mutation variant allele frequency (VAF) was determined by automated interpolation of the area under the curve and expressed as the ratio between the mutant peak area and the sum of mutant and wild-type peak areas × 100.CXCR4 expression analyses were done on blood collected in EDTA tubes and incubated with fluorochrome-labeled antibodies as described [12].
In summary, we found type 1 CALR mutation is more common in the more severe MTMD category, i.e. overt MF, is associated with lower platelet and higher LDH concentrations, larger spleen size, and earlier development of leukopenia and thrombocytopenia compared with type 2 mutations.Some but not all of these correlations are reported by others [13][14][15].We also found a strong correlation between type 1 CALR mutation VAF and indicators of disease progression, especially anemia and thrombocytopenia.These data reinforce the impact of type 1 CALR mutation on disease phenotype and trajectory, even though we did not find any influence on survival or progression to leukemia.
In our hypothesis-driven analyses, we first provide evidence of a dependence of CD34/CXCR4 expression on the CALR mutation type.Our study is retrospective and observational, so we can only infer causality between type 1 CALR gene expression and reduced CXCR4 expression.However, the association of type 1CALR mutation and low CD34/CXCR4 expression remained after adjusting for other co-variates associated with decreased CXCR4 regulation.Furthermore, type 1 CALR mutation VAF and down-regulated CD34/ CXCR4 expression respected a dose-response relationship.These characteristics of the results give support to the causality.This study suffers from limitations.Not all cases had all parameters useful for the analysis.However, our study is of a consecutive series of cases from an institutional database that systematically collects the most important disease co-variates.
Our study provides an innovative perspective on how CD34/CXCR4 may be differentially regulated in type1 CALR-mutated MTMD, a finding with potential implications for predicting prognosis and for therapy.Comparative studies through targeted and methylation sequencing can further clarify the epigenetic diversity between type 1 and 2 CALR-mutated people with MTMD.

Table 1 .
Clinical and laboratory characteristics of myelofibrosis-type megakaryocyte dysplasia (MTMD) subjects stratified according to the CALR mutation subtypes.
P = 0.94 Data refers to the diagnosis of the disease, i.e. at the time of the diagnostic bone marrow biopsy.IQR Interquartile range, LDH lactic dehydrogenase, UPN upper limit of normal.